PCR Troubleshooting and Optimization: The Essential Guide

Quantitative PCR data analysis, unlocking the secret to successful results (ISBN: 978-1-904455-72-1)

Abstract

Real-time quantitative PCR (qPCR) is the gold standard for fast, accurate, sensitive and costefficient gene expression analysis. Despite its conceptual simplicity and ease of use, the multi-step qPCR workflow contains many potential pitfalls. An intelligent experiment design and setup, high quality reagents and assays, quality controls in each step of the workflow, proper quantification models and appropriate bio-statistical analyses pave the way to successful gene expression results. This chapter will cover all data analysis aspects from the evaluation of pilot studies and quality controls, through universally applicable quantification models and bio-statistics, to the reporting of experiment results.

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