Learn all about post qPCR quality control

In a 50 minute video I will walk you through the different qPCR data quality control steps:

  • detection of abnormal amplification
  • inspection of melting curves
  • PCR efficiency evaluation
  • determination of reference gene expression stability
  • control on PCR replicate variation
  • assessment of positive and negative controls
  • evaluation of deviating sample normalization factors
  • quality control on inter run calibration

Headshot of Bram De Craene - qPCR expert Biogazelle

Bram De Craene obtained his PhD in Sciences (Biotechnology) at Ghent University in 2005.
As a postdoc he got skilled in a broad range of molecular techniques to study the process of epithelial-mesenchymal transition (EMT) during cancer progression. He joined Biocartis in December 2014 as technical lead to design and develop in vitro diagnostic tests.

As a consultant with Biogazelle he supports the qPCR analysis software qbase+ and he is trainer of (inter)national qPCR courses.

Watch the free training