In a 50 minute video I will walk you through the different qPCR data quality control steps:
- detection of abnormal amplification
- inspection of melting curves
- PCR efficiency evaluation
- determination of reference gene expression stability
- control on PCR replicate variation
- assessment of positive and negative controls
- evaluation of deviating sample normalization factors
- quality control on inter run calibration
Bram De Craene obtained his PhD in Sciences (Biotechnology) at Ghent University in 2005.
As a postdoc he got skilled in a broad range of molecular techniques to study the process of epithelial-mesenchymal transition (EMT) during cancer progression. He joined Biocartis in December 2014 as technical lead to design and develop in vitro diagnostic tests.
As a consultant with Biogazelle he supports the qPCR analysis software qbase+ and he is trainer of (inter)national qPCR courses.
